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AccuZol� Total RNA Extraction Solution
RNA Extraction � AccuZol� Total RNA Extraction Solution
AccuZol� is a ready-to-use reagent for the isolation of total RNA from various sample materials.
A monophasic solution of phenol and guanidine-salt inhibits RNase activity, and so the reagent maintains integrity of the RNA during sample homogenization or lysis.
AccuZol� allows you to extract high-yield total RNA with small quantities of starting materials.
Features and Benefits
Simplicity
AccuZol� minimize the steps handle hazardous reagents so that RNase/DNase contamination keeps to a minimum.
Experiment Time
The procedure for total RNA isolation can be completed in 1 hour
Sample Source
Tissues and cultured cells originated from human, animals, plant, and bacteria, or blood samples.
Easy Phase Separation
Green colored reagent is useful to distinguish between organic phase and aqueous phase.
Application
cDNA synthesis, RT-PCR, Real-time RT-PCR, Northern/dot blot analysis, RNase protection assays, Molecular cloning
Specifications
|
Samples |
Starting volume |
Elution volume |
Average yield (ug) |
Animal Tissue |
Liver
Kidney
Spleen
Lung
Brain |
10 mg |
50ul |
50 - 70
25 - 40
40 - 60
15 - 25
8 - 12 |
Cultured Cell |
HeLa
A549
PC3 |
1 x 106 cells |
50ul |
15 - 30
15 - 25
15 - 25 |
Bacterial Cell |
E.coli |
1 x 107 cells |
50ul |
10 - 15 |
Plant Tissue |
Bean leaf |
100 mg |
50ul |
30 - 50 |
Blood |
Human whole blood |
250 ul |
50ul |
2 - 3 |
Applications
cDNA synthesis, Northern blotting, RT-PCR, real time RT-PCR
Billing information
Catalog number : K-3090
Quantity: 100 extractions
Availability: Yes
Price
365 €
Order: Total RNA Extraction Solution
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